Derivatization Techniques for Chromatographic Analysis

New approach to derivatization and gas-chromatographic analysis of barbiturates.

A recently developed procedure for alkylatiori of organic acids has proven extremely successful for preparation of volatile alkyl derivatives of the barbiturates for gas-chromatographic analysis. Soluble salts are formed in a mixture of N,N-dimethylacetamide and methanol. These in turn react rapidly with alkyl iodides to form the corresponding alkyl derivatives. The butyl derivatives of barbitu...

A Sensitive Liquid Chromatographic Method for the Analysis of Clarithromycin with Pre-Column Derivatization: Application to a Bioequivalence Study

Objective(s) A sensitive liquid chromatographic method for the analysis of clarithromycin- a macrolide antibiotic- in human serum, using pre-column derivatization with 9-fluorenylmethyl chloroformate (FMOC-Cl) is described. Materials and Methods The method involved liquid-liquid extraction of the drug and an internal standard (amantadine) followed by pre-column derivatization of the analytes...

a sensitive liquid chromatographic method for the analysis of clarithromycin with pre-column derivatization: application to a bioequivalence study

objective(s) a sensitive liquid chromatographic method for the analysis of clarithromycin- a macrolide antibiotic- in human serum, using pre-column derivatization with 9-fluorenylmethyl chloroformate (fmoc-cl) is described. materials and methods the method involved liquid-liquid extraction of the drug and an internal standard (amantadine) followed by pre-column derivatization of the analytes wi...

Gas-chromatographic determination of valproic acid in serum without derivatization.

Valproic acid can be determined by gas-chromatography as the free acid after its extraction (1 ) or derivatization to form methyl, butyl, or phenacyl estars (2-4), the latter methods being more complicated for routine analyses. Other procedures, such as enzyme immunoassay (Syva Co., Palo Alto, CA 94304) or fluoroimmunoassay (Miles Inc., Elkhart, IN 46515), which do not require serum extraction,...

Analysis of Emulsifier in Food Using Chromatographic Techniques